Dasiel Oscar Borroto Escuela

Senior Lab Manager
Karolinska Institutet
E-mail: Dasiel.borroto.escuela@ki.se
Page: http://www.dasiel-lab.be

Fields of interest:

I have been primarily focused on understanding the molecular integration of signals in the brain via receptor-receptor interaction in the heteroreceptor complexes and its functional effects which is a current scientific issue of high relevance for brain function and integration. And whether alterations in specific heteroreceptor complexes and their receptor-receptor interactions are associated with and/or play a role in pathogenetic mechanisms contributing to brain disease development, inter alia Parkinson’s disease, schizophrenia, addiction and depression. I’m very excited by the tremendous potential of the field of heteroreceptor complexes in the CNS and their receptor-receptor interactions. They offer novel targets for treatment of psychiatry and neurological disorders. My small daily ambition is to be part of this novel exciting field in Neurosciences. And also to develop new methods to describe in a better way receptor-receptor interactions in the brain. I have also a strong interest in setting up and improve the newly developed in situ proximity ligation assays for identification and study of heteroreceptor complexes in the brain. Furthermore, on novel adaptations of conventional Light Resonance Energy Transfer-based methods (BRET, FRET, TR-FRET, BiFC) to made possible the study GPCR oligomerization dynamics in heterologous expression systems and neurons.

Recent publications:

Borroto-Escuela DO, Hinz S, Navarro G, Franco R, Müller CE, Fuxe K.Understanding the Role of Adenosine A2AR Heteroreceptor Complexes in Neurodegeneration and Neuroinflammation. Front Neurosci. 2018 Feb 6;12:43. doi: 10.3389/fnins.2018.00043. eCollection 2018.

Feltmann K, Borroto-Escuela DO, Rüegg J, Pinton L, de Oliveira Sergio T, Narváez M, Jimenez-Beristain A, Ekström TJ, Fuxe K, Steensland P.Effects of Long-Term Alcohol Drinking on the Dopamine D2 Receptor: Gene Expression and Heteroreceptor Complexes in the Striatum in Rats. Alcohol Clin Exp Res. 2018 Feb;42(2):338-351. doi: 10.1111/acer.13568.

Borroto-Escuela DO, Wydra K, Li X, Rodriguez D, Carlsson J, Jastrzębska J, Filip M, Fuxe K.Disruption of A2AR-D2R Heteroreceptor Complexes After A2AR Transmembrane 5 Peptide Administration Enhances Cocaine Self-Administration in Rats. Mol Neurobiol. 2018 Jan 30. doi: 10.1007/s12035-018-0887-1.

Hernández-Pérez OR, Crespo-Ramírez M, Cuza-Ferrer Y, Anias-Calderón J, Zhang L, Roldan-Roldan G, Aguilar-Roblero R, Borroto-Escuela DO, Fuxe K, Perez de la Mora M. Differential activation of arginine-vasopressin receptor subtypes in the amygdaloid modulation of anxiety in the rat by arginine-vasopressin. Psychopharmacology (Berl). 2018 Jan 7. doi: 10.1007/s00213-017-4817-0.

Navarro G, Borroto-Escuela D, Angelats E, Etayo Í, Reyes-Resina I, Pulido-Salgado M, Rodríguez-Pérez AI, Canela EI, Saura J, Lanciego JL, Labandeira-García JL, Saura CA, Fuxe K, Franco R. Receptor-heteromer mediated regulation of endocannabinoid signaling in activated microglia. Role of CB1 and CB2 receptors and relevance for Alzheimer’s disease and levodopa-induced dyskinesia. Brain Behav Immun. 2018 Jan;67:139-151. doi: 10.1016/j.bbi.2017.08.015.

Beggiato S, Borelli AC, Borroto-Escuela D, Corbucci I, Tomasini MC, Marti M, Antonelli T, Tanganelli S, Fuxe K, Ferraro L. Cocaine modulates allosteric D2-σ1 receptor-receptor interactions on dopamine and glutamate nerve terminals from rat striatum. Cell Signal. 2017 Dec;40:116-124. doi: 10.1016/j.cellsig.2017.09.007.

Borroto-Escuela DO, DuPont CM, Li X, Savelli D, Lattanzi D, Srivastava I, Narváez M, Di Palma M, Barbieri E, Andrade-Talavera Y, Cuppini R, Odagaki Y, Palkovits M, Ambrogini P, Lindskog M, Fuxe K. Disturbances in the FGFR1-5-HT1A Heteroreceptor Complexes in the Raphe-Hippocampal 5-HT System Develop in a Genetic Rat Model of Depression. Front Cell Neurosci. 2017 Oct 10;11:309. doi: 10.3389/fncel.2017.00309. eCollection 2017.

Rivera A, Gago B, Suárez-Boomgaard D, Yoshitake T, Roales-Buján R, Valderrama-Carvajal A, Bilbao A, Medina-Luque J, Díaz-Cabiale Z, Craenenbroeck KV, Borroto-Escuela DO, Kehr J, Rodríguez de Fonseca F, Santín L, de la Calle A, Fuxe K. Dopamine D4 receptor stimulation prevents nigrostriatal dopamine pathway activation by morphine: relevance for drug addiction. Addict Biol. 2017 Sep;22(5):1232-1245. doi: 10.1111/adb.12407.

Borroto-Escuela DO, Li X, Tarakanov AO, Savelli D, Narváez M, Shumilov K, Andrade-Talavera Y, Jimenez-Beristain A, Pomierny B, Díaz-Cabiale Z, Cuppini R, Ambrogini P, Lindskog M, Fuxe K. Existence of Brain 5-HT1A-5-HT2A Isoreceptor Complexes with Antagonistic Allosteric Receptor-Receptor Interactions Regulating 5-HT1A Receptor Recognition. ACS Omega. 2017 Aug 31;2(8):4779-4789. doi: 10.1021/acsomega.7b00629.

Millón C, Flores-Burgess A, Narváez M, Borroto-Escuela DO, Gago B, Santín L, Castilla-Ortega E, Narváez JÁ, Fuxe K, Díaz-Cabiale Z. The neuropeptides Galanin and Galanin(1-15) in depression-like behaviours. Neuropeptides. 2017 Aug;64:39-45. doi: 10.1016/j.npep.2017.01.004.