Roberto Fernandez-Lafuente

Fields of interest:

Enzyme immobilization, enzyme stabilization, design of bioprocess.

Recent publications:

Tacias-Pascacio, V.G., Virgen-Ortíz, J.J., Jiménez-Pérez, M., Yates, M., Torrestiana-Sanchez, B., Rosales-Quintero, A., Fernandez-Lafuente, R. Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: Critical role of the immobilization support. (2017) Fuel, 200, pp. 1-10.

Zaak, H., Siar, E.-H., Kornecki, J.F., Fernandez-Lopez, L., Pedrero, S.G., Virgen-Ortíz, J.J., Fernandez-Lafuente, R. Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads.(2017) Process Biochemistry, 56, pp. 117-123.

Albuquerque, T.L.D., Rueda, N., Dos Santos, J.C.S., Barbosa, O., Ortiz, C., Binay, B., Özdemir, E., Gonçalves, L.R.B., Fernandez-Lafuente, R. Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment(20 16) Process Biochemistry, 51 (7), pp. 865-874

Sanchez, A., Cruz, J., Rueda, N., Dos Santos, J.C.S., Torres, R., Ortiz, C., Villalonga, R., Fernandez-Lafuente, R.Inactivation of immobilized trypsin under dissimilar conditions produces trypsin molecules with different structures (2016) RSC Advances, 6 (33), pp. 27329-27334.

Tacias-Pascacio, V.G., Peirce, S., Torrestiana-Sanchez, B., Yates, M., Rosales-Quintero, A., Virgen-Ortíz, J.J., Fernandez-Lafuente, R. Evaluation of different commercial hydrophobic supports for the immobilization of lipases: Tuning their stability, activity and specificity (2016) RSC Advances, 6 (102), pp. 100281-100294.

Peirce, S., Virgen-Ortíz, J.J., Tacias-Pascacio, V.G., Rueda, N., Bartolome-Cabrero, R., Fernandez-Lopez, L., Russo, M.E., Marzocchella, A., Fernandez-Lafuente, R. Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase(2016) RSC A dvances, 6 (66), pp. 61707-61715.

Santos, J.C.S.D., Barbosa, O., Ortiz, C., Berenguer-Murcia, A., Rodrigues, R.C., Fernandez-Lafuente, R. Importance of the Support Properties for Immobilization or Purification of Enzymes (2015) ChemCatChem, 7 (16), pp. 2413-2432.

Rueda, N., Dos Santos, J.C.S., Torres, R., Ortiz, C., Barbosa, O., Fernandez-Lafuente, R. Improved performance of lipases immobilized on heterofunctional octyl-glyoxyl agarose beads  (2015) RSC Advances, 5 (15), pp. 11212-11222. Cited 32 times.

Barbosa, O., Ortiz, C., Berenguer-Murcia, Á., Torres, R., Rodrigues, R.C., Fernandez-Lafuente, R. Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts(2015) B iotechnology Advances, 33 (5), pp. 435-456.

Dos Santos, J.C.S., Rueda, N., Barbosa, O., Fernández-Sánchez, J.F., Medina-Castillo, A.L., Ramón-Márquez, T., Arias-Martos, M.C., Millán-Linares, M.C., Pedroche, J., Yust, M.D.M., Gonçalves, L.R.B., Fernandez-Lafuente, R. Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin (2015) RSC Advances, 5 (27), pp. 20639-20649.